Basic Science Support Research Paper Kit Development… “Replaces the existing high-cost test method”
(Daejeon = Yonhap News) Reporter Jeong Yoon-deok = A kit has been developed that can detect life-threatening so-called’super bacteria’ right away due to resistance to antibiotics.
Hangu Basic Science Research Institute announced on the 15th that Dr. Choi Jong-soon of the Materials Analysis Research Department has developed a multi-detection kit (hereinafter referred to as mPAD) that accurately detects Clostridioides difficile (hereinafter referred to as C. difficile), the highest threat super bacteria.
C. difficile, which leads to death in severe cases, does not have a clear treatment, so early diagnosis is required to prevent the spread of infection.
However, the currently used test method takes a lot of time and has a very low sensitivity because it takes a lot of time and has a very low sensitivity because it takes a lot of time and is performed in three stages, from an antigen test to a patient’s feces, a toxin test, and a genetic test.
The research team created a hydrophilic and hydrophobic pattern on paper through wax printing and stacked them in five layers to produce an mPAD with a three-dimensional fluid passage.
If a fecal sample of a patient suspected of being infected with mPAD is dropped, it is possible to check with the naked eye at the same time whether one C. difficile biomarker antigen and two toxins are detected within 10 minutes.
A trace amount of low-concentration C. difficile sample can also be detected in a maximum of 1 hour through high-sensitivity signal amplification.
As a result of evaluating the performance of mPAD by Professor Dal-Sik Kim’s team of Chonbuk National University Medical School, the detection sensitivity was 97%, the specificity was 88%, and the accuracy was 95%.
The mPAD is made of paper and has the advantage of being user-friendly because it is inexpensive to manufacture and does not require additional equipment.
Dr. Joseph Kwon, who participated in the development, said, “A study that suggested the possibility of securing and localizing C. Difficile diagnostic technology,” and said, “Through this achievement, we provide an accurate and inexpensive C. Difficile diagnostic method to replace the existing test method including expensive genetic testing. I can do it,” he said.
A paper containing such research results was published in the authoritative journal’Biosensors and Bioelectronics’ in the field of analytical chemistry.
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2021/03/15 10:06 sent