The highest threat level super bacteria detected in 10 minutes… 95% accuracy

Basic Science Support Research Paper Kit Development… “Replaces the existing high-cost test method”

Superbacteria detection experiment results using mPAD

In the top, C. difficile was not detected, in the middle, one antigen was detected, and in the bottom, two toxins were detected. [한국기초과학지원연구원 제공. 재판매 및 DB 금지]

(Daejeon = Yonhap News) Reporter Jeong Yoon-deok = A kit has been developed that can detect life-threatening so-called’super bacteria’ right away due to resistance to antibiotics.

Hangu Basic Science Research Institute announced on the 15th that Dr. Choi Jong-soon of the Materials Analysis Research Department has developed a multi-detection kit (hereinafter referred to as mPAD) that accurately detects Clostridioides difficile (hereinafter referred to as C. difficile), the highest threat super bacteria.

C. difficile, which leads to death in severe cases, does not have a clear treatment, so early diagnosis is required to prevent the spread of infection.

However, the currently used test method takes a lot of time and has a very low sensitivity because it takes a lot of time and has a very low sensitivity because it takes a lot of time and is performed in three stages, from an antigen test to a patient’s feces, a toxin test, and a genetic test.

The research team created a hydrophilic and hydrophobic pattern on paper through wax printing and stacked them in five layers to produce an mPAD with a three-dimensional fluid passage.

mPAD multilayer structure
mPAD multilayer structure

It is designed so that the sample flows into both channels first, and then the reagent flows to amplify the detection signal. [한국기초과학지원연구원 제공. 재판매 및 DB 금지]

If a fecal sample of a patient suspected of being infected with mPAD is dropped, it is possible to check with the naked eye at the same time whether one C. difficile biomarker antigen and two toxins are detected within 10 minutes.

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A trace amount of low-concentration C. difficile sample can also be detected in a maximum of 1 hour through high-sensitivity signal amplification.

As a result of evaluating the performance of mPAD by Professor Dal-Sik Kim’s team of Chonbuk National University Medical School, the detection sensitivity was 97%, the specificity was 88%, and the accuracy was 95%.

The mPAD is made of paper and has the advantage of being user-friendly because it is inexpensive to manufacture and does not require additional equipment.

mPAD development research team
mPAD development research team

From left to right: Choi Jong-soon, Kwon Joseph, and Dr. Do-Kyung Han [한국기초과학지원연구원 제공. 재판매 및 DB 금지]

Dr. Joseph Kwon, who participated in the development, said, “A study that suggested the possibility of securing and localizing C. Difficile diagnostic technology,” and said, “Through this achievement, we provide an accurate and inexpensive C. Difficile diagnostic method to replace the existing test method including expensive genetic testing. I can do it,” he said.

A paper containing such research results was published in the authoritative journal’Biosensors and Bioelectronics’ in the field of analytical chemistry.

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