PAC-MANN Assay Detects Early-Stage Pancreatic Cancer with High Accuracy

by drbyos
Credit: iStock/© Eraxion

PAC-MANN: A Revolutionary Blood Test for Early Pancreatic Cancer Detection

Researchers at Oregon Health & Science University (OHSU) have unveiled a new, non-invasive, high-throughput blood test designed to identify early-stage pancreatic ductal adenocarcinoma (PDAC). Called PAC-MANN (Protease-ACtivated MAgnetic NaNosensor), this innovative assay offers hope for more effective screening and early intervention against one of the deadliest cancers.

Understanding pancreatic cancer: A silent killer

Pancreatic cancer, specifically pancreatic ductal adenocarcinoma (PDAC), is notorious for its aggressive nature and insidious onset. Unlike many other cancers, PDAC rarely shows symptoms until it has advanced significantly, making early detection a major challenge.

The breakthrough: PAC-MANN assay

Senior author Jared Fischer, PhD, and his team from the Cancer Early Detection Advanced Research Center at OHSU developed PAC-MANN, a blood test that can identify PDAC by measuring protease activity. Proteases, enzymes that break down proteins, play a pivotal role in cancer progression by enabling tumor cells to invade surrounding tissues and spread to new locations.

How PAC-MANN works

The PAC-MANN assay screens for proteases, specifically members of the matrix metalloproteinase (MMP) family, using peptide probes that emit a fluorescent signal when cleaved. This process allows the test to distinguish between healthy individuals and those with PDAC, showing impressive results even in early-stage disease.

Performance metrics

In a recent study, PAC-MANN identified PDAC samples with a sensitivity of 73% and specificity of 98%, outperforming the FDA-approved CA 19-9 test commonly used to monitor disease progression. When combined, the two methods achieved a sensitivity of 87% and specificity of 96% for PDAC detection. Notably, the assay reached an astounding 85% sensitivity for stage I disease.

Beyond standard screening

While current tests focus on the general population, PAC-MANN targets high-risk groups, including individuals with pancreatitis, diabetes, and a family history of PDAC. These individuals lack effective monitoring tools, making PAC-MANN a crucial step forward in cancer surveillance.

Future directions

The researchers are optimistic about the potential of PAC-MANN, indicating plans for a multi-center clinical trial. They are also working on expanding the panel of peptide probes to improve assay performance and exploring its use as a treatment response monitor. Given its low cost and simplicity, PAC-MANN could reach underserved and rural areas, offering hope to patients worldwide.

Moving towards clinical translation

Translating PAC-MANN from research to clinical practice involves FDA approval and well-designed trials. The team remains committed to bringing this groundbreaking technology within reach, emphasizing the importance of a comprehensive clinical evaluation.

Conclusion

The PAC-MANN assay represents a significant advancement in the battle against pancreatic cancer. By enabling early detection and personalized treatment strategies, this innovative test could save countless lives. As research progresses, PAC-MANN stands poised to become a vital tool in the fight against one of the most devastating cancers.

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